Mass spectrometry is an analytical technique that ionizes the sample into a gaseous ion mixture and separates it according to the mass charge ratio (m/z). The simplest use of mass spectrometry is molecular weight measurement (click to enter). The characteristic fragment ions can also be used for qualitative analysis of analytes. In addition, the quantitative analysis of analytes can be carried out according to the peak strength of the mass spectrum.
Ion source: a device that ionizes the atoms or molecules of the analyzed sample into charged particles (ions), and accelerates the ions to enter the analyzer. According to the different ionization methods, the following are commonly used for the analysis of biological macromolecules:
1. ESI (Electrospray Ionization): electric spray ionization - the softest ionization mode
2. MALDI (Matrix Assisted Laser Desorption): Matrix Assisted Laser Desorption ionization
| Molecular weight analysis | Protein Mass Spectrometry | Technical service of liquid mass spectrometry |
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Molecular weight is one of the key characteristic parameters of protein and the most basic physical and chemical property parameter of organic compounds. The correct molecular weight often represents the correct structure of the organic compounds and biological macromolecules measured. Molecular weight is also the primary parameter in the identification of peptides and proteins. With the advance of genome and proteome research, the scientific research of protein molecular weight measurement has attracted more and more attention. The following method accurately determines the molecular weight of the protein by ESI-TOF. The instrument Mass Spectrometer TripleTOF 5600 Manufacturer AB Science Instruments is also used in conjunction with other molecular weight mass spectrometry detection methods, and the results are further confirmed.
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Mass Spectrum (MS) is a technology to separate compounds into ions and fragment ions according to different mass charge ratios (m/s) for composition and structure analysis. The results obtained will be displayed in mass spectrum. We then carry out structural analysis of biological macromolecules, qualitative and quantitative analysis of organic and inorganic substances according to the information provided by the mass spectrum. Bio mass spectrometry (Bio MS) is a mass spectrometry protein sequencing technology used to analyze biological macromolecules. As we all know, biological macromolecules are mostly different from inorganic and organic small molecules by high molecular weight. For the latter, mass spectrometry detection only requires measuring the relative molecular weight of tens to 2000, while biological mass spectrometry technology requires tens of thousands or even hundreds of thousands of relative molecular weight. Therefore, mass spectrometry detection technology suitable for inorganic and organic small molecule analysis cannot be applied to the analysis of biological macromolecules. With the improvement and maturity of ESI and matrix assisted laser desorption ionization (MALDI) technology, mass spectrometry detection and analysis of biomacromolecules can be realized. |
Liquid chromatography-mass spectrometry (LC-MS) is a separation and analysis method that combines the high separation ability of HPLC and the high sensitivity and selectivity of MS with the mass spectrometer as the detection means. Especially in recent years, with the maturity of soft ionization technologies such as electrospray and atmospheric pressure chemical ionization, the qualitative and quantitative analysis results of sample protein sequencing are more reliable. At the same time, due to the unique advantages of liquid chromatography-mass spectrometry in the separation and identification of high boiling point, non-volatile and thermally unstable compounds, it has become the analysis, pharmacokinetics Food safety testing and clinical pharmaceutical research are indispensable means. |
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